The Role and Mechanism of M2c Macrophages in Lipopolysaccharide/D-Galactosamine Induced Acute Hepatitis

The study was based on the evaluation of positive effect M2c macrophages liver tissue inflammation, pathological changes and damage in lipopolysaccharide/D-galactosamine induced acute hepatitis. Meanwhile, whether anti-inflammatory effects through Janus kinase/signal transducer activator transcription signaling pathway also investigated. Forty institute cancer research female mice same batch with no significant difference body weight were distributed randomly into 5 different groups namely control group, solvent lipopolysaccharide/D-galactosamine, lipopolysaccharide/D-galactosamine+M2c lipopolysaccharide/ D-galactosamine+M2c+ruxolitinib, 8 each group. 6 h after last treatment serum collected to detect content alanine aminotransferase aspartate transferase. pathology determined using hematoxylineosin staining. determination levels expression factors, pro-inflammatory related apoptosis proteins carried out by Western blot. After induction function (alanine aminotransferase, aminotransferase), score slices, factors (interleukin-6, tumor necrosis factor-alpha, interleukin-1beta interleukin-10) significantly improved blood. Comparative normal apoptosis-related (B-cell lymphoma-extra-large, B-cell lymphoma 2, Bcl-2- associated X protein) other indicators higher a (p<0.05) under hepatitis model infusion from tail vein mice. above indexes exhibited statistically (p<0.05). adding ruxolitinib intervene basis function, slice score, pro factor protein expressions as compared group (p<0.05), while observed differences not (p>0.05) D-galactosamine Lipopolysaccharide/D-galactosamine induces occurrence hepatitis, which can well mimic virus pathogenic process critical role via activating pathway.